European Court judges have now concluded it was most likely Charlie was “being exposed to continued pain, suffering and distress” and undergoing experimental treatment with “no prospects of success… would offer no benefit”.
They said the application presented by the parents was “inadmissible” and said the court’s decision was “final”.
The court “also considered that it was appropriate to lift the interim measure” which had required doctors to continue providing life support treatment to Charlie.
BBC health correspondent Fergus Walsh said it is likely Charlie’s life support machine will be turned off within a few days following discussions between the hospital and his family.
Charlie’s parents, from Bedfont, west London, raised £1.3m on a crowdfunding site to pay for the experimental treatment in the US.
Ms Yates had already indicated the money would go towards a charity for mitochondrial depletion syndromes if Charlie did “not get his chance”.
“We’d like to save other babies and children because these medications have been proven to work and we honestly have so much belief in them.
“If Charlie doesn’t get this chance, we will make sure that other innocent babies and children will be saved”, she said.
In April a High Court judge ruled against the trip to America and said Charlie should be allowed to die with dignity.
Three Court of Appeal judges upheld the ruling in May and three Supreme Court justices dismissed a further challenge by the parents.